SHAPE analysis of the RNA secondary structure of the Mouse Hepatitis Virus 5' untranslated region and N-terminal nsp1 coding sequences.
Identifieur interne : 001250 ( Main/Exploration ); précédent : 001249; suivant : 001251SHAPE analysis of the RNA secondary structure of the Mouse Hepatitis Virus 5' untranslated region and N-terminal nsp1 coding sequences.
Auteurs : Dong Yang [États-Unis] ; Pinghua Liu [États-Unis] ; Elyse V. Wudeck [États-Unis] ; David P. Giedroc [États-Unis] ; Julian L. Leibowitz [États-Unis]Source :
- Virology [ 1096-0341 ] ; 2015.
Descripteurs français
- KwdFr :
- ARN viral (), ARN viral (métabolisme), Animaux, Conformation d'acide nucléique, Protéines virales non structurales (génétique), Protéines virales non structurales (métabolisme), Régions 5' non traduites (génétique), Régulation de l'expression des gènes viraux (physiologie), Souris, Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (métabolisme).
- MESH :
- génétique : Protéines virales non structurales, Régions 5' non traduites, Virus de l'hépatite murine.
- métabolisme : ARN viral, Protéines virales non structurales, Virus de l'hépatite murine.
- physiologie : Régulation de l'expression des gènes viraux.
- ARN viral, Animaux, Conformation d'acide nucléique, Souris.
English descriptors
- KwdEn :
- 5' Untranslated Regions (genetics), Animals, Gene Expression Regulation, Viral (physiology), Mice, Murine hepatitis virus (genetics), Murine hepatitis virus (metabolism), Nucleic Acid Conformation, RNA, Viral (chemistry), RNA, Viral (metabolism), Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (metabolism).
- MESH :
- chemical , chemistry : RNA, Viral.
- chemical , genetics : 5' Untranslated Regions, Viral Nonstructural Proteins.
- genetics : Murine hepatitis virus.
- metabolism : Murine hepatitis virus, RNA, Viral, Viral Nonstructural Proteins.
- physiology : Gene Expression Regulation, Viral.
- Animals, Mice, Nucleic Acid Conformation.
Abstract
SHAPE technology was used to analyze RNA secondary structure of the 5' most 474 nts of the MHV-A59 genome encompassing the minimal 5' cis-acting region required for defective interfering RNA replication. The structures generated were in agreement with previous characterizations of SL1 through SL4 and two recently predicted secondary structure elements, S5 and SL5A. SHAPE provided biochemical support for four additional stem-loops not previously functionally investigated in MHV. Secondary structure predictions for 5' regions of MHV-A59, BCoV and SARS-CoV were similar despite high sequence divergence. The pattern of SHAPE reactivity of in virio genomic RNA, ex virio genomic RNA, and in vitro synthesized RNA was similar, suggesting that binding of N protein or other proteins to virion RNA fails to protect the RNA from reaction with lipid permeable SHAPE reagent. Reverse genetic experiments suggested that SL5C and SL6 within the nsp1 coding sequence are not required for viral replication.
DOI: 10.1016/j.virol.2014.11.001
PubMed: 25462342
Affiliations:
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The structures generated were in agreement with previous characterizations of SL1 through SL4 and two recently predicted secondary structure elements, S5 and SL5A. SHAPE provided biochemical support for four additional stem-loops not previously functionally investigated in MHV. Secondary structure predictions for 5' regions of MHV-A59, BCoV and SARS-CoV were similar despite high sequence divergence. The pattern of SHAPE reactivity of in virio genomic RNA, ex virio genomic RNA, and in vitro synthesized RNA was similar, suggesting that binding of N protein or other proteins to virion RNA fails to protect the RNA from reaction with lipid permeable SHAPE reagent. Reverse genetic experiments suggested that SL5C and SL6 within the nsp1 coding sequence are not required for viral replication. </div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Indiana</li><li>Texas</li></region></list><tree><country name="États-Unis"><region name="Texas"><name sortKey="Yang, Dong" sort="Yang, Dong" uniqKey="Yang D" first="Dong" last="Yang">Dong Yang</name></region><name sortKey="Giedroc, David P" sort="Giedroc, David P" uniqKey="Giedroc D" first="David P" last="Giedroc">David P. Giedroc</name><name sortKey="Leibowitz, Julian L" sort="Leibowitz, Julian L" uniqKey="Leibowitz J" first="Julian L" last="Leibowitz">Julian L. Leibowitz</name><name sortKey="Liu, Pinghua" sort="Liu, Pinghua" uniqKey="Liu P" first="Pinghua" last="Liu">Pinghua Liu</name><name sortKey="Wudeck, Elyse V" sort="Wudeck, Elyse V" uniqKey="Wudeck E" first="Elyse V" last="Wudeck">Elyse V. 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